Use of calcium carbonate filler is the process called " alkaline sizing " and uses different chemistry than acid sizing.
2.
Quality of products can be evaluated using standard molecular methods such as quantification by spectrophotometry ( ThermoFisher NanoDrop ) or fluorimetry ( Tecan F200 fluorimeter ), nucleic acid size evaluation using gel and microfluidic chip electrophoresis ( BioRad Experion analyzer and Agilent Bioanalyzer ).
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