| 1. | Cloning vectors in yeast include yeast artificial chromosomes ( YACs ).
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| 2. | They are often used as a cloning vector in genetic engineering.
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| 3. | One bacterial plasmid used in genetic engineering as a plasmid cloning vector is pUC18.
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| 4. | Genome size varies among different organisms and the cloning vector must be selected accordingly.
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| 5. | P1 was developed as a cloning vector by Nat Sternberg and colleagues in the 1990s.
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| 6. | A plasmid cloning vector is typically used to clone DNA fragments of up to 15 kbp.
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| 7. | This method requires that restriction maps of the cloning vector and the insert are already available.
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| 8. | His cloning vectors were also used to develop the method for oligonucleotide site-directed mutagenesis.
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| 9. | There are many types of cloning vectors, but the most commonly used ones are genetically engineered plasmids.
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| 10. | Formation of recombinant DNA requires a cloning vector, a DNA molecule that replicates within a living cell.
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