The indicators quin - 2 , indo - land fura - 2 have the advantage of wavelength - shift upon binding ca2 + but share a common drawback in that the excitation bands for these indicators occur at uv wavelength ( 340 - 360nm ) , where autofluorescence from cells is high , radiation - damage of cellular structures is serious , the transmission of microscope optics is poor , and the availability of aberration - corrected optics for confocal microscopy is limited
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