Tagging can be done in various ways, such as nick translation, or PCR using tagged nucleotides.
2.
This process is called nick translation because the DNA to be processed is treated with DNase to produce single-stranded " nicks ".
3.
The structural and chemical aspects of this process, generally referred to as'nick translation', exceed the scope of this article.
4.
"' Nb . BbvCI "'is a fluorochrome-labeled nucleotides into specific spots of a DNA sequence via nick translation.
5.
Nick translation could cause double-stranded DNA breaks, if DNA polymerase I encounters another nick on the opposite strand, resulting in two shorter fragments.
6.
Nick translation is a biological process in which a single stranded DNA nick serves as the marker for DNA polymerase to excise and replace possibly damaged nucleotides.
7.
They can be used for strand-displacement amplification, Nicking Enzyme Amplification Reaction, exonucleotyic degradation, the creation of small gaps, or nick translation.
8.
Nick translation is used to label the DNA and involves cutting DNA and substituting nucleotides labelled with fluorophores ( direct labelling ) or biotin or oxigenin to have fluophore conjugated antibodies added later ( indirect labelling ).
9.
A DNA polymerase may perform this replacement via nick translation, a terminal excision reaction by its 5'--> 3'exonuclease activity, followed by a fill-in reaction by its polymerase activity.
10.
Therefore, the interpretation of these " in situ " assays of DNA fragmentation " in situ " nick translation ( ISNT ), TUNEL assay must be carefully assessed together with morphological features of apoptotic cells.
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