When a similar cluster of abnormalies appears in the absence of exposure to antifolates it is referred to as aminopterin-like syndrome without aminopterin.
12.
In contrast, animals given aminopterin in an amount of 0.041 mg / kg / day x 6 days without folic acid died.
13.
Similar congenital abnormalities have been documented with methotrexate, and collectively their teratogenic effects have become known as the " fetal aminopterin syndrome ".
14.
The use of aminopterin in cancer treatment was supplanted in the 1950s by methotrexate due to the latter's better therapeutic index in a rodent tumor model.
15.
Aminopterin is widely used in selection media ( such as HAT medium ) for cell culture, particularly in the development of hybridomas, which secrete monoclonal antibodies.
16.
This research subsequently led to the synthesis of the antifolate aminopterin, the first-ever anticancer drug, the clinical efficacy was proven by Sidney Farber in 1948.
17.
After fusion, the cells are grown in a medium with methotrexate or aminopterin that inhibit the enzyme dihydrofolate reductase thus blocking the de novo synthesis of thymidine monophosphate.
18.
As his condition improved, Pinkel began working with eminent researcher Sidney Farber in Boston, experimenting with aminopterin, which could induce temporary remissions in some leukemia cases.
19.
Fused cells are incubated in HAT medium ( hypoxanthine-aminopterin-thymidine medium ) for roughly 10 to 14 days . Aminopterin blocks the pathway that allows for nucleotide synthesis.
20.
Fused cells are incubated in HAT medium ( hypoxanthine-aminopterin-thymidine medium ) for roughly 10 to 14 days . Aminopterin blocks the pathway that allows for nucleotide synthesis.
