In the next step, an AP endonuclease creates a nick at the 5'end of the AP site, generating a hanging deoxyribose phosphate ( dRP ) residue in place of the AP site.
12.
Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another.
13.
Human AP Endonuclease ( APE1 ), like most AP endonucleases, is of class II and requires an Mg 2 + in its active site in order to carry out its role in base excision repair.
14.
This is due to demonstrated interactions with human AP endonuclease, though interactions between recombinant CSB and E . coli endonuclease IV as well as human N-terminus AP endonuclease fragments have not been observed in vitro.
15.
This is due to demonstrated interactions with human AP endonuclease, though interactions between recombinant CSB and E . coli endonuclease IV as well as human N-terminus AP endonuclease fragments have not been observed in vitro.
