Three of these were of the wild type : the apoenzyme in, the enzyme plus its magnesium ion cofactor in, and the enzyme at high ionic strength in.
12.
The processing protein, " MMAA " protein, fills the important role of aiding cofactor loading and exchange . " MMAA " protein favors association with the MCM apoenzyme, and allows for the transfer of the AdoCbl cofactor to the enzyme active site.
13.
1, 10-Phenanthroline is an inhibitor of metallopeptidases, with one of the first observed instances reported in carboxypeptidase A . Inhibition of the enzyme occurs by removal and chelation of the metal ion required for catalytic activity, leaving an inactive apoenzyme . 1, 10-Phenanthroline targets mainly zinc metallopeptidases, with a much lower affinity for calcium.
14.
An optic-fibre-based hydrogel-entrapped biosensor for glucose was made in the lab of Loeb ( Liao and colleagues ) and was named Sencil . this sensor was composed of a photocrosslinked diacrylate-modified PEG hydrogel containing the tetra-rhodamine ( TRITC ), labelled competitor betacyclodextrin and the quantum dot-labelled apoenzyme Concanavalin A . This sensor was tested only in vitro for functionality; however, some tests were done to see the compatibility of the fibre implanted transdermally in mice.
