Typically, Phrap had no problems differentiating between the different Alu copies in a cosmid, and to correctly assemble the cosmids ( or, later, BACs ).
12.
Typically, Phrap had no problems differentiating between the different Alu copies in a cosmid, and to correctly assemble the cosmids ( or, later, BACs ).
13.
After the construction of recombinant lambda or cosmid libraries the total DNA is transferred into an appropriate " E . coli " host via a technique called in vitro packaging.
14.
These extracts will recognize and package the recombinant molecules in vitro, generating either mature phage particles ( lambda-based vectors ) or recombinant plasmids contained in phage shells ( cosmids ).
15.
In his post-doctoral research he constructed the first reliable method for cloning human DNA cosmids-at the time an extremely difficult but rewarding accomplishment that has since been adopted by many other laboratories.
16.
Fluorescence " in situ " hybridization ( FISH ) with DNA clones ( YAC clones, cosmids ) allowed the construction of chromosome maps at a resolution of several megabases that could detect relatively small chromosome rearrangements.
17.
The fosmid system is excellent for rapidly creating chromosome-specific mini-BAC libraries from flow-sorted chromosomal DNA . The major advantage of Fosmids over other cosmid systems lies in its capability of stably propagating human DNA fragments.
18.
Cloning is generally first performed using " Escherichia coli ", and cloning vectors in " E . coli " include plasmids, bacteriophages ( such as phage ? ), cosmids, and bacterial artificial chromosomes ( BACs ).
19.
Cosmids can contain 37 to 52 ( normally 45 ) replicate as plasmids if they have a suitable origin of replication : for example SV40 ori in mammalian cells, ColE1 ori for double-stranded DNA replication or f1 ori for single-stranded DNA replication in prokaryotes.
20.
According to J . W . IJdo, " We conclude that the locus cloned in cosmids c8.1 and c29B is the relic of an ancient telomere-telomere fusion and marks the point at which two ancestral ape chromosomes fused to give rise to human chromosome 2 ."
