The internucleosomal DNA cleavage pattern was observed as a specific feature of apoptosis in 1978 / 1980 and has become a recognised hallmark of programmed cell death since then.
12.
It is the partial repeat sequence that prevents the CRISPR-Cas system from targeting the chromosome as base pairing beyond the spacer sequence signals self and prevents DNA cleavage.
13.
The briefly attached, covalently bonded TOP1-DNA structure at the 3'end of a cleaved DNA single strand is called a TOP1-DNA cleavage complex, or TOP1cc.
14.
The amino terminus of the alpha helix portion of zinc fingers targets the major grooves of the DNA helix and binds near the CCR5 gene positioning FokI in a suitable location for DNA cleavage.
15.
More recently, several structures of the DNA-bound structure have been solved in an attempt to understand both the chemical mechanism for DNA cleavage and the structural basis for inhibition of topoisomerase by antibacterial poisons.
16.
Although ribozymes mostly catalyze cleavage of RNA substrates, variety of reactions can be catalyzed by DNAzymes including RNA / DNA cleavage, RNA / DNA ligation, amino acid phosphorylation and dephosphorylation, and carbon carbon bond formation.
17.
The catalytic tyrosine lies on this WHD . The Toprim fold is a Rossmann fold that contains three invariant acidic residues that coordinate magnesium ions involved in DNA cleavage and DNA religation ( Avarind, Leipe, Konin, Nucleic Acids Research 1998 ).
18.
Artificial restriction enzymes created by linking the " Fok " I DNA cleavage domain with an array of DNA binding proteins or zinc finger arrays, denoted zinc finger nucleases ( ZFN ), are a powerful tool for host genome editing due to their enhanced sequence specificity.
19.
The enzyme "'FokI "', naturally found in " Flavobacterium okeanokoites ", is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal.
20.
A major advance is the creation of artificial restriction enzymes created by linking the FokI DNA cleavage domain with an array of DNA binding proteins or zinc finger arrays, denoted now as zinc finger nucleases ( ZFN ) . ) ZFNs are a powerful tool for host genome editing due to their enhanced sequence specificity.
