PstI is a useful enzyme for DNA cloning as it provides a selective system for generating hybrid DNA molecules.
12.
When the DNA clone is at or close to the mutant allele, the recombination frequency should be close to zero.
13.
At Incyte, order fulfillment is directed by technician Amy Ganschinietz, who helps prepare about 300 DNA clones on a typical day.
14.
Tissue engineering, DNA cloning, gene therapy and robots that perform delicate surgical procedures all broke through to scientific reality in 1997.
15.
The original DNA cloning patents awarded to Cohen and Boyer, the first of which was number 4, 237, 224, will expire next year.
16.
In that 1892 book, he posited a wild-eyed inventor who dreamed up ideas that prefigured the fax machine, the photocopier and DNA cloning.
17.
For each new DNA clone a polymorphism is identified and tested in the mapping population for its recombination frequency compared to the mutant phenotype.
18.
That conversation almost 25 years ago sparked a frenetic collaboration, and within three months the two scientists had demonstrated that DNA cloning was possible.
19.
DNA cloning, shotgun sequencing and site-directed mutagenesis became widely used to sequence large DNA molecules like human chromosomes and to engineer genes and proteins.
20.
Researchers order DNA clones through a Web site that lists a mind-numbing collection of offerings that would mean little to anyone outside the biotech industry.
