For duplex DNA nonexchangeable protons the H6 / H8 protons on the base correlate to their counterparts on neighboring bases and to the H1'proton on the sugar, allowing sequential walking to be done.
12.
Early reports on social media report high levels of'1D'accuracy ( sequencing one strand of the duplex DNA ),'2D'accuracy ( sequencing both the template and complement strand ) and assembled accuracy.
13.
The RuvAB complex can carry out DNA helicase activity, which helps unwind the duplex DNA . The binding of the RuvC protein to the RuvAB complex is thought to cleave the DNA strands, thereby resolving the Holliday junction.
14.
Intermolecular triplexes are formed between triplex-forming oligonucleotides ( TFO ) and target sequences on duplex DNA . Intramolecular triplexes are the major elements of H-DNAs, unusual DNA structures that are formed in homopurine-homopyrimidine regions of supercoiled DNAs.
15.
It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA . Two forms of this enzyme exist as likely products of a gene duplication event.
16.
The process of duplicating DNA is called DNA replication, and it takes place by first unwinding the duplex DNA molecule, starting at many locations called DNA replication origins, followed by an unzipping process that unwinds the DNA as it is being copied.
17.
Alternatively, the D-loop may be converted into a Holliday junction by cutting of the D-loop and a second exchange of DNA strands; the Holliday junction can be converted into linear duplex DNA by cutting of the Holliday junction and ligation of the resultant nicks.
18.
MGME1 has a strong preference for cutting single stranded DNA, with weak activity on duplex DNA, and no activity on RNA . Moreover, MGME1 removes single stranded 5?flaps in reconstituted mitochondrial DNA replication assays where it is required to enable ligation of the newly synthesized strand.
19.
One is that the MAEB stem-loop is really a DNA-binding domain of a dimeric protein, and that the two subunits of this protein bind on opposite strands of the duplex DNA . However, two nucleotides at complementary positions on the 5'and 3'side of the stem are almost always purines.
20.
Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'- GGATG-3'recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site.
