In size exclusion chromatography, where the separation process is driven by entropy, it is not possible to increase the resolution attained by a column via temperature or solvent gradients.
12.
Second, proteins can be separated according to their size or molecular weight via size exclusion chromatography or by SDS-PAGE ( sodium dodecyl sulfate-polyacrylamide gel electrophoresis ) analysis.
13.
Gel permeation chromatography, a type of size exclusion chromatography, is an especially useful technique used to directly determine the molecular weight distribution parameters based on the polymer's hydrodynamic volume.
14.
The highest resolution length sorting, with length variation of < 10 %, has thus far been achieved by size exclusion chromatography ( SEC ) of DNA-dispersed carbon nanotubes ( DNA-SWNT ).
15.
In the context of a purification strategy, denaturing condition electrophoresis provides an improved resolution over size exclusion chromatography, but does not scale to large quantity of proteins in a sample as well as the late chromatography columns.
16.
The most common technique for measuring molecular mass used in modern times is a variant of high-pressure liquid chromatography ( HPLC ) known by the interchangeable terms of size exclusion chromatography ( SEC ) and gel permeation chromatography ( GPC ).
17.
Experimental approaches for characterizing polymers are also common, using Polymer characterization methods, such as size exclusion chromatography, Viscometry, Dynamic light scattering, and Automatic Continuous Online Monitoring of Polymerization Reactions ( ACOMP ) for determining the chemical, physical, and material properties of polymers.
18.
The literature associated with measurements made by MALS photometers is extensive . both in reference to batch measurements of particles / molecules and measurements following fractionation by chromatographic means such as size exclusion chromatography ( SEC ), reversed phase chromatography ( RPC ), and field flow fractionation ( FFF ).
19.
For example, if there is any overlap between the displacer and the protein of interest, these low molecular mass materials can be readily separated from the purified protein during post-displacement processing using standard size-based purification methods ( e . g . size exclusion chromatography, ultrafiltration ).
20.
For fluorescence-detection size-exclusion chromatography-based thermostability assay ( FSEC-TS ) the samples are heated in the same manner as in FastPP and CETSA and after centrifugation to clear away precipitate the supernatant is treated in the same manner as FSEC . Larger aggregates are seen in the void volume while the peak height for the protein of interest decreases when the unfolding temperature is reached.
