| 11. | Notably, theorists have shown that sequencing via exonuclease enzymes as described here is not feasible.
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| 12. | Yeast contains Rat1 and Xrn1 exonuclease.
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| 13. | By itself, the protein has 3'to 5'exonuclease activity and endonuclease activity.
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| 14. | Pol ? lacks 3'to 5'exonuclease activity and is a moderate fidelity polymerase.
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| 15. | Most contaminating DNA is degraded by the addition of a second single-strand specific exonuclease.
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| 16. | This process involves the exonuclease's catching up to the pol II and terminating the transcription.
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| 17. | The ? subunit possesses DNA polymerase activity and the ? subunit is a 3-5 exonuclease.
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| 18. | This delay gives time for the DNA to be switched from the polymerase site to the exonuclease site.
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| 19. | The exonuclease activity of Rat1 degrades the RNA strand and halts transcriptions upon catching up to the polymerase.
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| 20. | The mutations that have been observed are in the regions that affect the exonuclease domain and polymerase domains.
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