When this is complete, a single nick on the leading strand and several nicks on the lagging strand can be found.
12.
Priming occurs once at the origin on the leading strand and at the start of each Okazaki fragment on the lagging strand.
13.
The leading strand is the template strand that is being replicated in the same direction as the movement of the replication fork.
14.
During leading strand synthesis thioredoxin-gp5 and gp4 form a high affinity complex increasing overall polymerase processivity to around 5 kb.
15.
In these plots, a positive deviation from C corresponds to lagging strand and negative deviation from C corresponds to leading strand.
16.
There is a richness of guanine over cytosine and thymine over adenine in the leading strand and vice versa for the lagging strand.
17.
Originally, this asymmetric nucleotide composition was explained as a different mechanism used in DNA replication between the leading strand and lagging strand.
18.
Leading strand synthesis begins with the synthesis of a short RNA primer at the replication origin by the enzyme Primase ( DnaG protein ).
19.
On the leading strand, DNA polymerase can make a complementary DNA strand without any difficulty because it goes from 5'to 3 '.
20.
DNA Polymerase ? will continuously add nucleotides to the template stand therefore making leading strand synthesis require only one primer and has uninterrupted DNA polymerase activity.