Dr . Gordon and colleagues later combined laser capture microdissection, and functional genomics to characterize specified cell populations within the gastrointestinal tract, including multipotent stem cells.
12.
Prior to MALBAC, a single cell is isolated by various methods including laser capture microdissection, microfluidic devices, flow cytometry, or micro pipetting, then lysed.
13.
A fifth UV based technology uses standard glass slides coated with an inert energy transfer coating and a UV based laser microdissection system ( typically a Leica LMD or PALM Zeiss machine ).
14.
This led to development of laser capture microdissection ( LCM ), a technique that can isolate individual cell types individual cells, which gave rise to cDNA libraries of specific cell types.
15.
Another process follows gravity-assisted microdissection method that turns on gravity to collect samples in tube cap under the slide used ( used by ION LMD system, Jungwoo F & B ).
16.
Another closely related LCM process ( used by Leica ) cuts the sample from above and the sample drops via gravity ( gravity-assisted microdissection ) into a capture device below the sample.
17.
To prepare cells for chromosome microdissection, a scientist first treats them with a chemical that forces them into metaphase : a phase of the cell's life-cycle where the chromosomes are tightly coiled and highly visible.
18.
If a RPMA containing cell fraction lysates of a tissue / s is to be made, laser capture microdissection ( LCM ) or fine needle aspiration methods is used to isolate specific cells from a region of tissue microscopically.
19.
Unlike traditional methods that yield a mishmash of proteins, this " laser capture microdissection " isolates pure cells and, in seconds, transfers them to a special film that preserves the patterns proteins make as normal cells turn cancerous.
20.
Transport by gravity using an upright microscope ( called GAM, gravity-assisted microdissection ) or transport by " laser pressure catapult "; the most recent generation utilizes a technology based on laser induced forward transfer ( LIFT ).
