The Micropipette developed by Heinrich Schnitger, which is often referred to as " Eppendorf pipette " in laboratory usage, counts today in various designs as standard almost every biomedical laboratories.
12.
For instance, the cannulation of a single microvessel with a micropipette, the microvessel is perfused with a certain pressure, occluded downstream and then the velocity of some cells will be related to the permeability.
13.
To treat male infertility, a micropipette sucks as few as 100 sperm from a man's ejaculate and shoots a single one into the egg, which is then put into the womb or fallopian tubes.
14.
The third problem, that of obtaining electrodes small enough to record voltages within a single axon without perturbing it, was solved in 1949 with the invention of the glass micropipette electrode, which was quickly adopted by other researchers.
15.
To administer the test, a police officer simply has to break a seal on a tiny micropipette of chemicals, and insert a particle of the suspected substance; if the chemicals turn purple, this indicates the possibility of marijuana.
16.
While glass micropipette electrodes measure the sum of the currents passing through many ion channels, studying the electrical properties of a single ion channel became possible in the 1970s with the development of the patch clamp by Erwin Neher and Bert Sakmann.
17.
And where Wilmut used an electric shock to insert the nucleus into a recipient egg whose only nucleus had been removed, the Hawaii researchers used a special micropipette to inject the donor nucleus into the mouse egg cell to create the new embryo.
18.
The free acid form of the dyes ( i . e . without the acetoxymethyl ester modification ) can also be directly injected into cells via a microelectrode or micropipette which removes uncertainties as to the cellular compartment holding the dye ( the acetoxymethyl ester can also enter the endoplasmic reticulum and mitochondria ).
19.
With this technique, a microelectrode was placed inside the motor endplate of the muscle fiber, and a micropipette filled with acetylcholine ( ACh ) is placed directly in front of the endplate in the synaptic cleft . A positive voltage was applied to the tip of the micropipette, which caused a burst of positively charged ACh molecules to be released from the pipette.
20.
With this technique, a microelectrode was placed inside the motor endplate of the muscle fiber, and a micropipette filled with acetylcholine ( ACh ) is placed directly in front of the endplate in the synaptic cleft . A positive voltage was applied to the tip of the micropipette, which caused a burst of positively charged ACh molecules to be released from the pipette.
