Determining dietary protein requirements using nitrogen balance requires that all nitrogen inputs and losses are carefully collected, to ensure that all nitrogen exchange is accounted for.
12.
A 70 kg adult human who was in the middle of the range would require approximately 45 grams of protein per day to be in nitrogen balance.
13.
Because of these conditions, it can be difficult to study the dietary protein requirements of certain populations using the nitrogen balance technique ( e . g . children ).
14.
The resulting positive nitrogen balance and alterations in the bacterial flora of the gut are likely to have beneficial effects in patients, helping to alleviate the symptoms of Crohn s.
15.
But Campbell found that the participants who consumed just the recommended amount of protein were not in nitrogen balance; to meet their bodies'needs, they needed more protein than they took in.
16.
In addition to measurements relating directly to skeletal muscle strength and mass, indirect measurements were made that demonstrated that all Skylab crewmembers had a negative nitrogen balance indicative of skeletal muscle attrition.
17.
The NBI ( Nitrogen Balance Index ) by Force-A, allows the assessment of nitrogen conditions of a culture by calculating the ratio between Chlorophyll and Flavonols ( related to Nitrogen / Carbon allocation ).
18.
Also, the study by Poullain et al ., which is often cited to demonstrate the superiority of whey protein hydrolysate by marketers, measured nitrogen balance in rats after three days of starvation, which corresponds to a longer period in humans.
19.
In order to control nitrogen inputs and losses, nitrogen balance studies usually require participants to eat very specific diets ( so total nitrogen intake is known ) and stay in the study location for the duration of the study ( to collect all nitrogen losses ).