In a second, much slower, step luciferin is regenerated from the oxidised ( oxyluciferin ) form, allowing it to recombine with aequorin, in readiness for a subsequent flash.
12.
Similarly, other results have indicated that the microenvironment of luciferase can force oxyluciferin into a more rigid, high-energy structure, forcing it to emit a high-energy green light.
13.
Luciferase activity is additionally inhibited by oxyluciferin and activated by ATP . When ATP binds to the enzyme s two allosteric sites, luciferase s affinity to bind ATP in its active site increases.
