In combination with the Alternative pathway hemolytic assay ( " AH50 " ) it can indicate terminal pathway deficiencies ( C3, C5-C9; absence of hemolysis in both CH50 and AH50 ), classic pathway deficiencies ( C1, C2, C4; absence of lysis in CH50 ) and alternative pathway deficiencies ( Factor I, B, H, D, properdin; absence of lysis in AH50 ).
