The glutamate residue is highly conserved; because, it both stabilizes the semiquinone form of FAD and is a proton donor / acceptor in the reaction.
12.
Then, the levopimaradiene goes through several more steps of oxidation processes, using oxygen molecules as the oxidizing agent, and NADPH as the proton donor.
13.
Both mechanisms exhibit the same distance between the proton donor and the glycosidic oxygen, situating the proton donor close enough to the glycosidic oxygen for hydrogen bonding.
14.
Both mechanisms exhibit the same distance between the proton donor and the glycosidic oxygen, situating the proton donor close enough to the glycosidic oxygen for hydrogen bonding.
15.
Mechanisms of catalysis include catalysis by bond strain; by proximity and orientation; by active-site proton donors or acceptors; covalent catalysis and quantum tunnelling.
16.
Later in the pathway, His263 is used as a proton donor for the substrate acceptor-TPP complex, which can then generate erythrose-4-phosphate.
17.
Crystal structures of DyP family members reveal two Asp most likely acts as a proton donor / acceptor and takes the place of the catalytic histidine used by plant peroxidases.
18.
These contain a glutamic acid residue which, on the basis of similarities with other families of glycosyl hydrolases, probably acts as the proton donor in their catalytic mechanism.
19.
In a hydrogen bond, the electronegative atom not covalently attached to the hydrogen is named proton acceptor, whereas the one covalently bound to the hydrogen is named the proton donor.
20.
Plasmepsins are aspartic acid proteases, meaning their active site contains two aspartic acid residue act respectively as proton donor and proton acceptor, catalysing the hydrolysis of peptide bond in proteins.
