Ashdown's medium, a selective medium containing gentamicin, may be required for cultures taken from nonsterile sites . " Burkholderia cepacia " medium may be a useful alternative selective medium in nonendemic areas, where Ashdown's is not available.
12.
Ashdown's medium, a selective medium containing gentamicin, may be required for cultures taken from nonsterile sites . " Burkholderia cepacia " medium may be a useful alternative selective medium in nonendemic areas, where Ashdown's is not available.
13.
Second, the isolate is cultured on mannitol salt agar, which is a selective medium with 7 9 % NaCl that allows " S . aureus " to grow, producing yellow-colored colonies as a result of mannitol fermentation and subsequent drop in the medium's pH.
14.
Upon transformation of the purified prey library with the bait plasmid, positive selection is achieved by growing the host E . coli on minimal medium lacking histidine ( NM selective medium ) that is usually supplemented with varying concentrations of 3-amino-triazole ( 3-AT ), a competitive inhibitor of HIS3.
15.
:I'm not sure how anyone could possibly answer the question as put; the quantity of medium you need would depend on the number of samples you're going to test, and on the lab protocol you're using for the testing ( I also wonder why you're using trypticase soy agar vs MacConkey medium-unless you're making your own selective medium, in which case again quantity depends on the recipe you're using .-Nunh-huh 02 : 44, 5 March 2008 ( UTC)
