If you have restriction enzyme markers at both ends of the gene, you can determine the approximate size of the gene using agarose gel electrophoresis, a process which in this context is called Southern blotting.
12.
Southern blotting, northern blotting, western blotting and Eastern blotting are molecular techniques for detecting the presence of microbial DNA sequences ( Southern ), RNA sequences ( northern ), protein molecules ( western ) or protein modifications ( Eastern ).
13.
DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.
14.
Southern blotting is the method used to determine the frequency of mitochondrial insertion in nDNA of the normal and the tumor cells of mice and rats, which proved that the mtDNA sequences are far more numerous and abundant in nDNA of rodent tumor cells in comparison with normal cells.
