| 11. | The albumin remains in the supernatant fraction during the solid / liquid extraction under these conditions.
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| 12. | Bottles with hemolytic supernatant were discarded.
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| 13. | The supernatant, known as clear juice is drawn off of the clarifier and sent to the evaporators.
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| 14. | There are many standardized cell lines available and supernatant is much simpler to analyze than proximal body fluid.
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| 15. | Next, phenanthridine is added to the supernatant and this is also mixed at 55 �C for 16 hours.
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| 16. | Purification of protein from bovine-derived stromal cell supernatants produces a substantially homogeneous factor, free of extraneous materials.
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| 17. | This maintains the well-being of the hybridomas and provides enough cells for cryopreservation and supernatant for subsequent investigations.
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| 18. | The supernatant water is then run back into the treatment process or disposed of as a waste-water stream.
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| 19. | This removes some of the salts present in the leftover supernatant and bound to DNA pellet making the final DNA cleaner.
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| 20. | The plasmid-containing supernatant is carefully removed and can be further purified or used for analysis, such as gel electrophoresis.
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