:Eliminative cleavage of beta-D-glucopyranosyl-( 1-> 4 )-beta-D-glucopyranosyluronate bonds of gellan backbone releasing tetrasaccharides containing a 4-deoxy-4, 5-unsaturated D-glucopyranosyluronic acid at the non-reducing end.
12.
Construction of a tetrasaccharide linker that consists of-GlcA?1 3Gal?1 3Gal?1 4Xyl?1-O-( Ser )-, where xylosyltransferase, ?4-galactosyl transferase ( GalTI ), ?3-galactosyl transferase ( GalT-II ), and ?3-GlcA transferase ( GlcAT-I ) transfer the four monosaccharides, begins synthesis of the GAG modified protein.
