Double-stranded RNA can also act as a catalytic, enzyme-dependent antisense agent through the RNAi / siRNA pathway, involving target mRNA recognition through sense-antisense strand pairing followed by target mRNA degradation by the RNA-induced silencing complex ( RISC ).
22.
In February 2010 researchers reported success in reducing HIV viral load using patient T-cells which had been harvested, modified with an RNA antisense strand to the HIV viral envelope protein, and re-infused into the patient during a planned lapse in retroviral drug therapy.
23.
Hence, a base triplet 3'- TAC-5'in the DNA antisense strand can be used as a template which will result in an 5'- AUG-3'base triplet in mRNA ( AUG is the codon for methionine, the start codon ).
