Retroviral aspartyl protease is synthesised as part of the POL polyprotein that contains; an aspartyl protease, a reverse transcriptase, RNase H and integrase.
22.
Methods for directly producing aspartyl-phenylalanine by enzymatic means, followed by chemical methylation, have also been tried, but not scaled for industrial production.
23.
Aspartyl proteases are a highly specific family of proteases-they tend to cleave dipeptide bonds that have hydrophobic residues as well as a beta-methylene group.
24.
This gene encodes a lysosomal aspartyl protease composed of a protein dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor.
25.
Aspartyl ( asp ) residues in the RNAP will hold on to Mg 2 + ions, which will, in turn, coordinate the phosphates of the ribonucleotides.
26.
Many eukaryotic P-type ATPases are monomeric or homodimeric enzymes of the catalytic subunit that hydrolyzes ATP . They contain the aspartyl phosphorylation site and catalyzes ion transport.
27.
In general, they have two highly conserved aspartates in the active site and are optimally active at acidic pH . Nearly all known aspartyl proteases are inhibited by pepstatin.
28.
High concentrations of isoleucine also result in the downregulation of aspartate s conversion into the aspartyl-phosphate intermediate, hence halting further biosynthesis of lysine, methionine, threonine, and isoleucine.
29.
They generally form a high-energy aspartyl-phosphoanhydride intermediate in the reaction cycle, and they interconvert between at least two different conformations, denoted by E 1 and E 2.
30.
A centrally located aspartyl residue in the 3rd TMS of the " Vibrio alginolyticus " homologue, conserved in all members of the family, has been shown to be essential for activity.
