:I'm assuming there are strept ? avidin beads like this being bound to biotinylated protein with varying numbers of moieties attached.
22.
For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.
23.
ProdiGene struck agreements with Sigma to distribute ProdiGene's corn-produced aprotinin, trypsin, beta-glucuronidase ( GUS ), and avidin.
24.
This allows elution from the avidin matrix to occur under milder, non-denaturing conditions, using low concentrations of biotin or low pH conditions.
25.
For a single high affinity biotin binding site without crosslinking, a monovalent version of avidin's distant relative, streptavidin, may be used.
26.
For example, avidin-displaying baculoviruses could be imaged in rat brain by coating them with biotinylated iron particles, rendering them visible in MR imaging.
27.
Avidin's affinity for biotin is exploited in wide-ranging biochemical assays, including western blot, ELISA, ELISPOT and pull-down assays.
28.
The non-reversible nature of the avidin-biotin complex can limit avidin s application in affinity chromatography applications where release of the captured ligand is desirable.
29.
Like avidin itself, NeutrAvidin is a tetramer with a strong affinity for biotin ( " K " d = 10 " 15 M ).
30.
Due to the high affinity of biotin to avidin and streptavidin, the radiotherapy accumulates where the antibody is, namely in the tumour, where it delivers its radioactivity.
