Subsequent work indicated that cleavage is actually driven by contraction of an actin filament network in the cortex rather than by expansion of the spindle but this idea was the forerunner of the current view of cytokinesis, in which interaction of spindle microtubules with the cell cortex determines the position of the cleavage furrow.
22.
The scaffold protein anillin is one of the most crucial partners of RhoA during cytokinesis and plays a fundamental role in the assembly and stabilization of the contractile ring by interacting with RhoA, septins, F-actin, myosin II, and mDia2 and it has been shown that its depletion results in cleavage furrow instability.
