Cloning vectors without promoter and RBS for the cloned DNA sequence are sometimes used, for example when cloning genes whose products are toxic to " genomic or cDNA library of clones since the cloned genes are normally subcloned into a more appropriate expression vector if their expression is required.
22.
But as Dr . Gerald Fink, a geneticist at the Whitehead Institute in Cambridge, Mass ., points out, it is not known how many other genes may play a role in the production and regulation of IAA . As a result, it remains to be seen how easily researchers can regulate growth using the newly cloned gene.
23.
The identity of this cloned gene cluster was corroborated by " kedA ", a gene in the cluster that encodes the previously isolated kedarcidin apoprotein, as well as " kedE " and " kedE10 ", the co-expression of which in " E . coli " led to the formation of a signature heptaene product previously implicated in enediyne core biosynthesis.
