When conducting DNA gel electrophoresis, crystal violet can be used as a nontoxic DNA stain as an alternative to fluorescent, intercalating dyes such as ethidium bromide.
22.
Iodide ( or ) interacts with and forms large complexes of crystal violet and iodine ( CV I ) within the inner and outer layers of the cell.
23.
Also in Gram staining, crystal violet stains only Gram-positive bacteria, and safranin counterstain is applied which stains all cells, even allowing the identification of Gram-negative bacteria as well.
24.
Because of presence of higher lipid content, after alcohol-treatment, the porosity of the cell wall increases, hence the CVI complex ( crystal violet iodine ) can pass through.
25.
The name " gentian violet " was originally used for a mixture of methyl pararosaniline dyes ( methyl violet ), but is now often considered a synonym for crystal violet.
26.
DNA stained with crystal violet can be viewed under natural light without the use of a UV transilluminator which is an advantage, however it may not produce a strong band.
27.
Staining a cell with a dye such as Giemsa stain or crystal violet allows a microscopist to describe its size, shape, internal and external components and its associations with other cells.
28.
The DNA is normally visualized by staining with ethidium bromide and then viewed under UV light, but other methods of staining are available, such as SYBR Green, GelRed, methylene blue, and crystal violet.
29.
Another example of a leuco dye is the crystal violet lactone, which in its lactone form is colorless or slightly yellowish, but in low pH, when it is protonated, it becomes intensely violet.
30.
However, if a large number of Heinz bodies are produced, as in the case of G6PD deficiency, some Heinz bodies will nonetheless be visible when viewing RBCs that have been stained with crystal violet.
