Thus, the electrophoretic mobility depends not only on the charge-to-mass ratio, but also on the physical shape and size of the protein.
22.
Charged analytes have a more complex interaction in the capillary because they exhibit electrophoretic mobility, engage in electrostatic interactions with the micelle, and participate in hydrophobic partitioning.
23.
Historically, the experimental techniques of choice to discover and analyze DNA binding sites have been the DNAse footprinting assay and the Electrophoretic Mobility Shift Assay ( EMSA ).
24.
The approach of MLST is distinct from Multi locus enzyme electrophoresis ( MLEE ), which is based on different electrophoretic mobilities ( EM ) of multiple core metabolic enzymes.
25.
Other biological macromolecules that can be analyzed with ELS include polysaccharides . pKa values of chitosans can be calculated from the dependency of electrophoretic mobility values on pH and charge density.
26.
Denaturing conditions are necessary for proper estimation of molecular weight of RNA . RNA is able to form more intramolecular interactions than DNA which may result in change of its electrophoretic mobility.
27.
It has been used in the electrophoretic mobility shift assay and p50 and title comp preincubated in assay buffer, pH 7.5 ( room temp ., 5 min ).
28.
Through combination with fine-control of pressure TRPS has been used to determine sample concentration and to accurately derive particle electrophoretic mobility & surface charge in addition to particle size information.
29.
Since the electroosmotic flow of the buffer solution is generally greater than that of the electrophoretic mobility of the analytes, all analytes are carried along with the buffer solution toward the cathode.
30.
It uses no charged dye so the electrophoretic mobility of proteins in CN-PAGE ( in contrast to the charge shift technique BN-PAGE ) is related to the intrinsic charge of the proteins.
