The network starts with fructose 6-phosphate ( F6P ), which has 6 carbon atoms with a label 13 C at carbon position 1 and 2.1, 2-13 C F6P becomes two glyceraldehyde 3-phosphate ( G3P ), one 2, 3-13 C T3P and one unlabeled T3P . The 2, 3-13 C T3P can now be reacted with sedoheptulose 7-phosphate ( S7P ) to form an unlabeled erythrose 4-phosphate ( E4P ) and a 5, 6-13 C F6P . The unlabeled T3P will react with the S7P to synthesize unlabeled products.
22.
It contains genes " rifG " through " rifN ", which were shown to biosynthesize AHBA . [ 10 ] " RifK ", " rifL ", " rifM ", and " rifN " are believed to act as transaminases in order to form the AHBA precursor kanosamine . " RifH " encodes aminoDAHP synthase that catalyzes the condensation between 1-deoxy-1-imino-d-erythrose 4-phosphate and phosphoenolpyruvate . " RifA " through " rifE " encode a type I polyketide synthase module, with the loading module being a non-ribosomal peptide synthase.
