| 21. | Because of its high quantum yield, B-PE is considered the world s brightest fluorophore.
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| 22. | If cleavage occurs, the fluorophore will be separated from the quencher molecule generating a detectable signal.
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| 23. | This enzyme has been found effective at improving organic fluorophore-stability in single-molecule experiments.
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| 24. | The choice of a particular fluorophore ultimately depends on the application and on its underlying photophysical properties.
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| 25. | Its fluorophore, and therefore its optical properties, are essentially identical to those of ethidium bromide.
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| 26. | Mathematical methods are used to restore spatio-temporal distribution of the thrombin from the fluorophore signal.
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| 27. | Another method of secondary antibody detection utilizes a near-infrared ( NIR ) fluorophore-linked antibody.
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| 28. | FRET will respond to internal conformational changes result from reorientation of the fluorophore with respect to the other.
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| 29. | For steady-state fluorescence measurements it is usually measured by embedding the fluorophore in a frozen polyol.
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| 30. | The antibody can either be conjugated to a fluorophore or to horseradish peroxidase for imaging and / or quantification.
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