First of all, Sutherland and Ted Rall were convinced that a sucrose homogenate of liver cells was absolutely necessary in order to keep their cells healthy and proliferating.
22.
It turned out that this sucrose was not necessary for the homogenate and once they set up the experiment without sucrose they were able to see more effective results.
23.
My other idea is that the homogenate was at 80?for all of the replicates, but that the loss of enzyme function was progressive the longer it was heated.
24.
The key to the success of this experiment was the use a homogenate of liver cells rather than intact liver cells, as they had been doing in their previous experiments.
25.
"In vitro ", including cell culture experiments, deiodination activity is determined by incubating cells or homogenates with high amounts of labeled thyroxine ( T 4 ) and required cosubstrates.
26.
The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis by Moore and Perez ( 1967 ).
27.
CA1 homogenates were probed with an antibody specific for Thr286 phosphorylated CaMKII and an antibody detecting total CaMKII . They observed a 22 % increase in CaMKII activity after LTP-inducing HFS.
28.
This inference was made by Rall from his experience studying mitochondria, which responded well to these sucrose homogenates; however, it had nothing to do with what was being studied at the time.
29.
The original assays for the detection of VGKC antibodies used Iodine-125 labelled dendrotoxin and the relatively mild detergent 2 % digitonin on mammalian brain homogenate, and VGKC with complexed proteins was extracted.
30.
Quantitative PCR was used to determine the abundance of PMMoV in raw sewage, treated wastewater, seawater exposed to wastewater, and fecal samples and / or intestinal homogenates from a wide variety of animals.
