In this case hypoxanthine-guanine phosphoribosyltransferase ( HGPRT ) reacts hypoxanthine absorbed from the medium with PRPP, liberating pyrophosphate, to produce IMP by a salvage pathway.
22.
In this case hypoxanthine-guanine phosphoribosyltransferase ( HGPRT ) reacts hypoxanthine absorbed from the medium with PRPP, liberating pyrophosphate, to produce IMP by a salvage pathway.
23.
Hypoxanthine, a breakdown product of ATP, contributes to the meat's flavor and odor, as do other products of the decomposition of muscle fat and protein.
24.
Fused cells are incubated in HAT medium ( hypoxanthine-aminopterin-thymidine medium ) for roughly 10 to 14 days . Aminopterin blocks the pathway that allows for nucleotide synthesis.
25.
Hypoxanthine and xanthine are two of the many bases created through mutagen presence, both of them through deamination ( replacement of the amine-group with a carbonyl-group ).
26.
The G-quartet typically binds monovalent cations such as potassium, while other bases can bind numerous other ligands such as hypoxanthine in a U-U-C-U quadruplex.
27.
Hypoxanthine is converted into guanine by the enzyme hypoxanthine-guanine phosphoribosyltransferase ( HGPRT ), while thymidine is phosphorylated by thymidine kinase ( TK ); both HGPRT and TK are enzymes of the salvage pathway.
28.
Hypoxanthine is converted into guanine by the enzyme hypoxanthine-guanine phosphoribosyltransferase ( HGPRT ), while thymidine is phosphorylated by thymidine kinase ( TK ); both HGPRT and TK are enzymes of the salvage pathway.
29.
The myeloma cells are selected beforehand to ensure they are not secreting antibody themselves and that they lack the hypoxanthine-guanine phosphoribosyltransferase ( HGPRT ) gene, making them sensitive to the HAT medium ( see below ).
30.
However, normal human and mouse cells can still multiply as they can utilize hypoxanthine and thymidine present in the medium through a salvage pathway, which ordinarily recycles the purines and pyrimidines produced from degradation of nucleic acids.
