During DNA replication of circular bacterial genomes, multiple proteins are involved in synthesizing the leading strand, and the Okazaki fragments on the lagging strand.
22.
In addition, the highly transcribed genes, such as ribosomal proteins, have been shown to be located mostly on the leading strand in bacteria.
23.
At the leading strand, loading of the PCNA is an infrequent process, because DNA replication on the leading strand is continuous until replication is terminated.
24.
At the leading strand, loading of the PCNA is an infrequent process, because DNA replication on the leading strand is continuous until replication is terminated.
25.
Phosphodiester links form between the 3'to 5'and nucleotides can be added with the aid of the enzyme DNA polymerase for the continuous leading strand.
26.
The leading strand is continuously extended from the primer by a DNA polymerase with high processivity, while the lagging strand is extended discontinuously from each primer forming Okazaki fragments.
27.
In eukaryotes, leading strand synthesis is thought to be conducted by Pol ?; however, this view has recently been challenged, suggesting a role for Pol ?.
28.
First, an RNA primer is synthesized by primase, and, like that in leading strand synthesis, DNA Pol III binds to the RNA primer and adds deoxyribonucleotides.
29.
The " oriC " site in " E . coli " has three primer for the leading strand and to add RNA primers on the lagging strand.
30.
This means that, while polymerisation of the leading strand proceeds, polymerisation of the lagging strand only occurs after enough of the lagging strand has been unwound by the helicase.
