At the bottom end of the separation tube, the effluent is mixed with ninhydrin, a chemical that changes color in the presence of amines, a component part of amino acids.
22.
Its peptide-like structure, including the presence of glycine and valine, was first suggested by a combination of acidic hydrolysis, acetylation, ninhydrin staining, and paper chromatography, among other experiments.
23.
If the amounts of amino acids are in excess of 10 nmol, ninhydrin can be used for this; it gives a yellow colour when reacted with proline, and a vivid purple with other amino acids.
24.
More advanced methods include DNA quantification, infrared spectroscopy . and for buried individuals changes in soils such as the levels of methane, phosphates and nitrates, ninhydrin-reactive nitrogen, volatile organic compounds and water conductivity.
25.
Whereas for most carbonyl compounds, a carbonyl form is more stable than a product of water addition ( hydrate ), ninhydrin forms a stable hydrate of the central carbon because of the destabilizing effect of the adjacent carbonyl groups.
26.
His goal came to fruition when, in 1950, he changed the name of the company to Pierce Chemical Company and his newly renamed company was the first commercial producer of ninhydrin, a chemical used in amino acid analysis.
27.
A solution suspected of containing the ammonium ion can be tested by ninhydrin by dotting it onto a solid support ( such as silica gel ); treatment with ninhydrin should result in a dramatic purple color if the solution contains this species.
28.
A solution suspected of containing the ammonium ion can be tested by ninhydrin by dotting it onto a solid support ( such as silica gel ); treatment with ninhydrin should result in a dramatic purple color if the solution contains this species.
29.
Note that to generate the ninhydrin chromophore ( 2-( 1, 3-dioxoindan-2-yl ) iminoindane-1, 3-dione ), the amine is condensed with a molecule of ninhydrin to give a Schiff base.
30.
Note that to generate the ninhydrin chromophore ( 2-( 1, 3-dioxoindan-2-yl ) iminoindane-1, 3-dione ), the amine is condensed with a molecule of ninhydrin to give a Schiff base.
