Where \ lambda is the wavelength of the fluorescent emission and NA is the numerical aperture of the microscope.
22.
High magnification objective lenses typically have higher numerical apertures ( and so better optical sectioning ) than low magnification objectives.
23.
Objective lenses with higher magnifications normally have a higher numerical aperture and a shorter depth of field in the resulting image.
24.
Simulations show that such aberration-free designs are applicable to high-numerical aperture lenses such as flat microscope objectives.
25.
A lens with a larger numerical aperture will be able to visualize finer details than a lens with a smaller numerical aperture.
26.
A lens with a larger numerical aperture will be able to visualize finer details than a lens with a smaller numerical aperture.
27.
In objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.
28.
Furthermore altering the size of the condenser diaphragm allows adjustment of sample depth of field by altering the effective numerical aperture of the microscope.
29.
Some of the design methods for nonimaging optics are also finding application in imaging devices, for example some with ultra-high numerical aperture.
30.
The lens needs to be large enough to support the full numerical aperture of the fiber, and must not introduce aberrations in the beam.
