As the Okazaki fragments are synthesized, the RNA primers are replaced with DNA nucleotides and the fragments are bonded together in a continuous complementary strand.
22.
During DNA replication of circular bacterial genomes, multiple proteins are involved in synthesizing the leading strand, and the Okazaki fragments on the lagging strand.
23.
This process is referred to as'maturation of Okazaki fragments', and ligase ( see below ) completes the final step in the maturation process.
24.
The viral DNA polymerase then uses a strand displacement mechanism, as opposed to the conventional Okazaki fragments used in mammalian DNA replication, to replicate the genome.
25.
There are two problems after leading and lagging strand synthesis : RNA remains in the duplex and there are nicks between each Okazaki fragment in the lagging duplex.
26.
While this process seems quite simple and repetitive, defects in Okazaki fragment maturation can cause DNA strand breakage which can cause varying forms of " chromosome aberrations ".
27.
The discontinuous stretches of DNA replication products on the lagging strand are known as Okazaki fragments and are about 100 to 200 bases in length at eukaryotic replication forks.
28.
The leading strand is continuously extended from the primer by a DNA polymerase with high processivity, while the lagging strand is extended discontinuously from each primer forming Okazaki fragments.
29.
The Okazakis'experiments provided extensive information on the replication process of DNA and the existence of short, newly synthesized DNA chains that later became known as Okazaki fragments.
30.
An enzyme called "'DNA ligase "'connects the gap in the backbone by forming a phosphodiester bond between each gap that separates the Okazaki fragments.
