They were originally isolated from mammalian cells, and named according to their electrophoretic mobility in polyacrylamide gels.
22.
Later, Rabilloud et al . used RuBPS as a fluorescent label for protein detection in polyacrylamide gels.
23.
Polyacrylamide gels are run in a vertical configuration while agarose gels are typically run horizontally in a submarine mode.
24.
The specifically bound DNA is eluted from each individual protein-DNA complex and analyzed by polyacrylamide gel electrophoresis.
25.
The native enzyme was shown to be made up of two subunit monomers via SDS-polyacrylamide gel electrophoresis.
26.
Alternatively, one may run the sample through an agarose or polyacrylamide gel, alongside some samples of known concentration.
27.
They also differ in their casting methodology, as agarose sets thermally, while polyacrylamide forms in a chemical polymerization reaction.
28.
Folded protein complexes of interest separate cleanly and predictably due to the specific properties and polymerization time of the polyacrylamide gel.
29.
Elchrom Scientific sells Spreadex gels which are precast, can be high throughput and are more sensitive than standard polyacrylamide gels.
30.
Lamanda et al . improved the RuBPS staining protocol by selectively destaining the polyacrylamide matrix while the protein content remained tinctured.
