Dynactin p27 C-terminal T186 residue is phosphorylated by cyclin-dependent kinase 1 ( Cdk1 ) in mitosis and helps target polo-like kinase 1 ( Plk1 ) to kinetochores during prometaphase.
22.
During prometaphase and metaphase this spindle attaches itself to the chromosomes at a particular point called the kinetochore and undergoes several growing and shortening periods in tuning with the back and forth oscillations of the chromosomes.
23.
At the onset of mitosis ( prophase, prometaphase ), the cellular machinery is engaged in the disassembly of various cellular components including structures such as the nuclear envelope, the nuclear lamina and the nuclear pore complexes.
24.
Early events of metaphase can coincide with the later events of prometaphase, as chromosomes with connected kinetochores will start the events of metaphase individually before other chromosomes with unconnected kinetochores that are still lingering in the events of prometaphase.
25.
Early events of metaphase can coincide with the later events of prometaphase, as chromosomes with connected kinetochores will start the events of metaphase individually before other chromosomes with unconnected kinetochores that are still lingering in the events of prometaphase.
26.
Although this process is not well understood, high-resolution imaging of live mouse oocytes has revealed that chromosomes form an intermediate chromosomal configuration, called the " prometaphase belt ", which occurs prior to biorientation.
27.
It is likely that, in animal cells, at least some of the activation of APC / C Cdc20 occurs early in the cell cycle ( prophase or prometaphase ) based on the timing of the degradation of its substrates.
28.
It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.
29.
Through this work it was found that, in mammalian cells, Kinesin-5 is required for the initial assembly of the mitotic spindle during prophase and prometaphase, but is dispensable to traverse subsequent anaphase during a round of mitosis.
30.
In one model, unattached prometaphase kinetochores can'sensitize'APC to inhibition of MCC by recruiting the APC to kinetochores via a functioning SAC . Furthermore, depletions of various SAC proteins have revealed that MAD2 and BUBR1 depletions affect the timing of mitosis independently of kinetochores, while depletions of other SAC proteins result in a dysfunctional SAC without altering the duration of mitosis.
