Extracellular miRNAs are capable of targeting mRNAs in the recipient cell through RNA interference pathways . " In vitro " experiments have shown the transfer of specific exRNAs into recipient cells inhibiting protein expression and preventing cancer cell growth.
22.
Extracellular miRNAs are capable of targeting mRNAs in the recipient cell through RNA interference pathways . " In vitro " experiments have shown the transfer of specific exRNAs into recipient cells inhibiting protein expression and preventing cancer cell growth.
23.
This finding suggests that " P . multocida " might be competent to undergo transformation under certain conditions, and that genome maintenance genes in transforming donor DNA may preferentially replace their damaged counterparts in the DNA of the recipient cell.
24.
Because the authors of this study did not find RNA-induced silencing complex-associated proteins in these exosomes, they suggested that only the pre-miRNAs but not the mature miRNAs in MSC exosomes have the potential to be biologically active in the recipient cells.
25.
During conjugation the donor cell provides a conjugative or mobilizable genetic element that is most often a plasmid or transposon . [ 4 ] [ 5 ] Most conjugative plasmids have systems ensuring that the recipient cell does not already contain a similar element.
26.
There is some debate within the scientific community over whether this cell can be considered completely synthetic on the grounds that the chemically synthesized genome was an almost 1 : 1 copy of a naturally occurring genome and, the recipient cell was a naturally occurring bacterium.
27.
I don't think it is realistic to expect that someone will try to synthesize an entire human genome and transplant it into a recipient cell for the purpose of tissue regeneration . . . we already have lots of experience with stem cells that will probably get there much faster.
28.
The steps they applied to build this was first they simulated a model of this genome computationally, they identified DNA via watermarks; next, they chemically produced this genome in the laboratory and finally, transplanted this genome into a recipient cell to produce a synthetic cell solely controlled by this synthetic genome.
29.
In the F-plasmid system the relaxase enzyme is called TraI and the relaxosome consists of TraI, TraY, TraM and the integrated host factor IHF . The nicked strand, or " T-strand ", is then unwound from the unbroken strand and transferred to the recipient cell in a 5'- terminus to 3'- terminus direction.
30.
The choice of recipient cell type is often based on an experimental need to examine the protein's function in detail, and the most prevalent recipients, known as "'heterologous expression systems "', are chosen usually because they are easy to transfer DNA into or because they allow for a simpler assessment of the protein's function.
