If polymerase chain reaction ( PCR ) tests on infected material are positive, subsequent restriction endonuclease pattern analysis of the amplified outer membrane protein A gene can be done to determine the genotype.
22.
Despite the uncertainty concerning the structure-catalysis relationship of type II endonucleases, site-directed mutagenesis of the restriction endonuclease HindIII has provided much insight into the key amino acid residues involved.
23.
Like RFLP, CAPS works on the principle that genetic differences between individuals can create or abolish restriction endonuclease restriction sites, and that these differences can be detected in the resulting DNA fragment length after digestion.
24.
Early research ( 1992 ) described two very similar, yet unique variants : HHV-6A and HHV-6B . The distinction was warranted due to unique restriction endonuclease cleavages, monoclonal antibody reactions, and growth patterns.
25.
After foreign DNA fragments, which have also been cut with the same restriction endonuclease, have been inserted into host cell, the restriction endonuclease gene is expressed by applying heat, or by introducing a biomolecule, such as arabinose.
26.
After foreign DNA fragments, which have also been cut with the same restriction endonuclease, have been inserted into host cell, the restriction endonuclease gene is expressed by applying heat, or by introducing a biomolecule, such as arabinose.
27.
If the recognition site is outside the region of the cleavage pattern, then the restriction endonuclease is referred to as Type I . If the recognition sequence overlaps with the cleavage sequence, then the restriction endonuclease restriction enzyme is Type II.
28.
If the recognition site is outside the region of the cleavage pattern, then the restriction endonuclease is referred to as Type I . If the recognition sequence overlaps with the cleavage sequence, then the restriction endonuclease restriction enzyme is Type II.
29.
If all of the digests produce fragments that add up one may infer the position of the REN ( restriction endonuclease ) sites by placing them in spots on the original DNA fragment that would satisfy the fragment sizes produced by all three digests.
30.
"Neisseria meningitides " has multiple type II restriction endonuclease systems that are employed in natural genetic transformation is a process by which a recipient bacterial cell can take up DNA from a neighboring donor bacterial cell and integrate this DNA into its genome by recombination.
