| 21. | Unlike the other types, type IV restriction enzymes recognize and cut only modified DNA.
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| 22. | Restriction enzymes digest portions of the DNA, leaving short fragments.
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| 23. | All three plasmids are digested with appropriate restriction enzyme and then allowed to ligate.
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| 24. | The second method employs the use of restriction enzymes and a recognized DNA sequence.
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| 25. | This would help digest sequences that are not being recognized by methylation-sensitive restriction enzymes.
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| 26. | Restriction enzymes are endonucleases from eubacteria and archaea that recognize a specific DNA sequence.
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| 27. | There are numerous types of restriction enzymes, each of which will cut DNA differently.
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| 28. | DNA is digested with the restriction enzyme AluI, many 1109 base-pair fragments were obtained.
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| 29. | The idea behind using the same restriction enzymes is to create ligation later on.
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| 30. | Other viruses, such as bacteriophages T3 and T7, encode proteins that inhibit the restriction enzymes.
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