| 21. | The second method employs the use of restriction enzymes and a recognized DNA sequence.
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| 22. | These standard sequences encode specific restriction enzyme sites.
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| 23. | All three plasmids are digested with appropriate restriction enzyme and then allowed to ligate.
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| 24. | Arber had discovered the type of restriction enzymes that dismantle genetic material at specific points.
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| 25. | Unlike the other types, type IV restriction enzymes recognize and cut only modified DNA.
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| 26. | The nucleotide sequence recognized for cleavage by a restriction enzyme is called the restriction site.
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| 27. | There are numerous types of restriction enzymes, each of which will cut DNA differently.
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| 28. | Simultaneously, the same restriction enzymes are used to digest ( cut ) the destination.
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| 29. | An organism's genomic DNA is extracted and then digested with a restriction enzyme.
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| 30. | It involves the use of restriction enzymes.
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