When a retrovirus enters a host cell's cytoplasm, it undergoes processes such as capsid uncoating and reverse transcription.
22.
Generally this is achieved through the use of reverse transcription of the RNA followed by polymerase chain reaction.
23.
This step does not have to be necessarily carried out before the ligation and reverse transcription steps outlined below.
24.
A direct confirmation can be obtained by reverse transcription polymerase chain reaction where the genome of the virus is amplified.
25.
It is predicted that reverse transcription is also negatively affected by APOBEC3G binding to viral RNA and causing steric alterations.
26.
Hepatitis B is one of a few known non-retroviral viruses which use reverse transcription as a part of its replication process.
27.
Within the cytoplasm the viral reverse transcriptase enzyme performs reverse transcription of the viral RNA genome to create a viral DNA genome.
28.
The first step in RACE is to use reverse transcription to produce a cDNA copy of a region of the RNA transcript.
29.
The product line includes various subbrands of biotechnology products, such as machines and consumables for polymerase chain reaction, reverse transcription, cloning, immunoassays.
30.
A cDNA library is constituted by the cDNA produced through reverse transcription of mRNA collected from specific cells of types of cell.
