Texas researcher Barton Kamen said one of the first drugs used in chemotherapy-- aminopterin, now no longer manufactured-- may be a useful treatment for children with acute lymphocytic leukemia who do not respond to the standard chemotherapeutic drug.
32.
Similar effects have been noted in rodent species as well, where the range for rescue by folic acid was fairly narrow and highly dependent on the timing ( optimal of 1 hour prior to aminopterin ) of administration in relation to aminopterin.
33.
Similar effects have been noted in rodent species as well, where the range for rescue by folic acid was fairly narrow and highly dependent on the timing ( optimal of 1 hour prior to aminopterin ) of administration in relation to aminopterin.
34.
1947 : Dr . Sidney Farber, pediatric pathologist, requested Dr . Yellapragada Subbarow ( of Lederle lab and his friend and colleague at Harvard Medical School ) to supply Aminopterin and later Amithopterin ( Methotrexate ) to conduct trials on acute leukemic children.
35.
In humans, leucovorin rescue at overdosages lower than 10 mg aminopterin in an average 70 kg adult should comprise an initial leucovorin dose of at least 20 mg ( 10.0 mg / m 2 ), given intravenously ( preferably ), or orally.
36.
Dr . Sidney Farber received the Lasker in 1966 for his 1947 discovery that a combination of aminopterin and methotrexate, both folic acid antagonists, could produce remission in patients with acute leukemia, and for " his constant leadership in the search for chemical agents against cancer ".
37.
Additionally, to prevent reversible aminopterin-mediated nephrotoxicity manifesting as increases in serum creatinine and which further delays drug elimination, urinary alkalinization with and volume expansion should be considered in cases of massive aminopterin overdosage, particularly those involving greater than 100 mg AMT in an average 70 kg adult human.
38.
Additionally, to prevent reversible aminopterin-mediated nephrotoxicity manifesting as increases in serum creatinine and which further delays drug elimination, urinary alkalinization with and volume expansion should be considered in cases of massive aminopterin overdosage, particularly those involving greater than 100 mg AMT in an average 70 kg adult human.
39.
On a HAT medium, only those cells that have active HGPRT ( HGPRT + ) and TK ( TK + ) enzymes can proliferate, while those deficient in these enzymes ( HGPRr-and / or TK-) can not divide ( since they cannot produce purines and pyrimidines due to the aminopterin present in the HAT medium ).
40.
Massive aminopterin overdosage in humans ( i . e . > 40 mg AMT in an average 70 kg adult ), should be approached with an initial leucovorin dose of 100 mg ( 50 mg / m 2 ), given intravenously and continued at 6 hour intervals until the hematological abnormalities are improved ( likely 8 12 courses or more ).
