Cloning vectors without promoter and RBS for the cloned DNA sequence are sometimes used, for example when cloning genes whose products are toxic to " genomic or cDNA library of clones since the cloned genes are normally subcloned into a more appropriate expression vector if their expression is required.
32.
This may be useful in TA cloning, whereby a cloning vector ( such as a plasmid ) that has a T ( thymine ) 3'overhang is used, which complements with the A overhang of the PCR product, thus enabling ligation of the PCR product into the plasmid vector.
33.
These unique features and other features gave significance of the T-even phages, this includes transduction which is responsible for transfer of drug resistant features, lysogenic conversion is responsible for acquisition of new characteristics such as the formation of new enzymes, random insertion into bacterial chromosome can induce insertional mutation, epidemiological typing of bacteria ( phage typing ), phages are used extensively in genetic engineering where they serve as cloning vectors.
