| 31. | The azide-tagged protein is then labeled with cyclooctyne-fluorophore conjugate to yield a fluorescently labeled protein.
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| 32. | They are labeled at their reducing ends with a fluorescent label ( i . e . a fluorophore ).
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| 33. | Therefore, the fluorophore used, the length of the linker, and its position must be carefully selected.
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| 34. | Light is directed at the fluorophore which absorbs energy and then re-emits light at a longer wavelength.
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| 35. | The technique relies on a distant-dependent transfer of energy from a donor fluorophore to an acceptor dye.
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| 36. | On one end of the pin is a chemical unit called a fluorophore, which can absorb and emit light.
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| 37. | When these two units touch _ when the clothespin is closed _ the quencher keeps the fluorophore from lighting up.
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| 38. | The antibodies are then coupled chemically to a fluorophore and used to trace the proteins in the cells under study.
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| 39. | Reabsorption happens because another molecule or part of a macromolecule absorbs at the wavelengths at which the fluorophore emits radiation.
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| 40. | If this is the case, some or all of the photons emitted by the fluorophore may be absorbed again.
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