The delivery of plasmids into rat neurons through the use of a gene gun, specifically DRG neurons, is also used as a pharmacological precursor in studying the effects of neurodegenerative diseases such as Alzheimer's disease.
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Dr . Harriman says that a few months ago, scientists here used what they call a gene gun to shoot a bacterial gene into a tissue of grass in the hope of making it immune to Roundup.
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A central instrumentation facility also lies within department having sophisticated instruments like DNA Sequencer, High performance liquid chromatography ( HPLC system ), Prep-HPLC system, LC-MS system, GC-MS system, Gene Gun, Flow cytometer, Fluorescent microscopes, Fermenter, RT-PCR machine etc.
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Laboratories around the country are aiming their gene guns at pig and goat cells in hopes of developing pharmaceutical proteins and transplantable organs for humans or injecting pollen grains with DNA to produce virus-resistant papayas, potatoes, tobacco and tomatoes.
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For some crops, such as corn, the scientists inserted traits into a single cell, using a gene gun that fires microscopic bullets of gold, coated with DNA . With others, they carried the gene into the cell with a common soil bacteria.
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TEXCOCO, Mexico ( AP ) _ In a laboratory set amid acres ( hectares ) of grain, Natasha Bohorova loads a tiny corn embryo and an invisible bit of genetic matter into a " gene gun, " a high-tech device resembling a microwave oven.
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Gene transfer into the genome of the desired organism, as for fish in this case, requires a vector like a lentivirus or mechanical / physical insertion of the altered genes into the nucleus of the host by means of a micro syringe or a gene gun.
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Although similar gene guns have been used for several years to fire DNA into plant cells, " these are the first experiments using a gene gun in tumor-bearing animals, " said Wenn H . Sun, who also is reporting the results in a scientific paper later this month.
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Although similar gene guns have been used for several years to fire DNA into plant cells, " these are the first experiments using a gene gun in tumor-bearing animals, " said Wenn H . Sun, who also is reporting the results in a scientific paper later this month.
